Journal article
Proteomic profiling of secretome and adherent plasma membranes from distinct mammary epithelial cell subpopulations
H Ji, RJA Goode, F Vaillant, S Mathivanan, EA Kapp, RA Mathias, GJ Lindeman, JE Visvader, RJ Simpson
Proteomics | WILEY-BLACKWELL | Published : 2011
Abstract
The stem cell niche comprises stem cells (SCs), stromal cells, soluble factors, extracellular matrix constituents and vascular networks. The ability to identify signals that regulate SC self-renewal and differentiation is confounded by the difficulty in isolating pure SC niche components in sufficient quantities to enable their biochemical characterisation. Here, we report the extracellular (secretome) and adherent plasma membrane proteomes of three distinct epithelial cell subpopulations isolated and immortalized from the mouse mammary gland - basal and mammary stem cell (basal/MaSC), luminal progenitor (LP) and mature luminal (ML) cell lines. GeLC-MS/MS-based proteomic profiling revealed a..
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Awarded by National Health & Medical Research Council of Australia (NHMRC)
Funding Acknowledgements
The authors are grateful to D. Galloway for providing plasmids. This work was supported by the National Health & Medical Research Council of Australia (NH&MRC) program grant #487922 (R.J.S.). Analysis of proteomic data described in this work was supported by the Australian Proteomics Computational Facility funded by the NH&MRC grant #381413. S.M. was supported by an NH&MRC Fellowship #1016599. R.G. was supported by an Australian Postgraduate Award. This work was supported by funds from the Operational Infrastructure Support Program provided by the Victorian Government Australia. They acknowledge the Australian Cancer Research Foundation for providing funds to purchase the Orbitrap (TM) mass spectrometer. J.V., G.J.L. and F.V. were supported by the NH&MRC and the Victorian Government, through Victorian Cancer Agency funding of the Victorian Breast Cancer Research Consortium.